The Concept Series Workstations have been recently remodelled. This report details the oxygen concentration throughout the work area within the workstation in anaerobic mode to ensure proper performance. These workstations will achieve less than 10 ppm Oxygen, and will return to the normal working oxygen level efficiently following operation of the sleeves, Single Plate Entry System (SPES), or interlock chamber.
Baker Ruskinn design and manufacture workstations for the anaerobic microbiology, cell biology, stem cell and regenerative medicine markets. These workstations have historically controlled temperature, humidity, Oxygen, and Carbon Dioxide concentration. As technology and science develops, our workstations will too, grow. This new upgraded model of the Concept line of workstations have been redesigned to meet and exceed these new expectations, one of which is to provide and maintain an anaerobic environment, where there is a lack of Oxygen. It is required for the Oxygen (O2) level to be less than 20 ppm (0.002%).
The Concept 400 workstation was connected to standard supply gasses – H2N2 and N2. The Southland Sensing OMD-501D Trace Oxygen Analyser, and Southland sensing TO2-2X PPM Oxygen Sensor were calibrated using N6 nitrogen gas (>99.9999% purity) and then placed inside the workstation.
The workstation was placed into Gas Control mode as per the user guide, and upon completion of the gas mixing cycle the first O2 measurement was recorded at the intervals of 0, 1, 2, 4 and 24 hours. Following the sample at 24 hours, operations of glove port entry, SPES and interlock were performed, and the maximum oxygen level was recorded, and the time taken to recover to pre-operation oxygen ppm levels was measured.
It can be demonstrated that: