Induced pluripotent stem (iPS) cells are generated from somatic cells by the forced expression of a defined set of pluripotency-associated transcription factors. Human iPS cells can be propagated indefinitely, while maintaining the capacity to differentiate into all cell types in the body except for extraembryonic tissues.
Each of these studies looks at a different cell type: MEF, Adipose-derived stem cells, liver bud, and hiPSC-EC in ischemic retinal conditions.
For MEF cells, results showed:
(Yoshida et al 2009, Cell Stem Cell: ‘Hypoxia Enhances the Generation of Induced Pluripotent Stem Cells’)
For adipose-derived stem cells, results showed:
(Shimada et al 2011, BBRC: ‘Accelerated generation of human induced pluripotent stem cells with retroviral transduction and chemical inhibitors under physiological hypoxia‘)
For liver bud cells, results showed:
(Ayabe et al 2018, Stem Cell Reports: ‘Optimal Hypoxia Regulates Human iPSC-Derived Liver Bud Differentiation through Intercellular TGFB Signaling’)
For hiPSC-EC in ischemic retinal conditions, results showed:
(Cho et al 2020, JCI: ‘iPSC-derived endothelial cell response to hypoxia via SDF1a/CXCR4 axis facilitates incorporation to revascularize ischemic retina’)
If these studies are of interest to you, have you considered signing up for our HypoxEU international forum on oxygenation and biology on September 16th? You can sign up to the virtual event here: https://zoom.us/meeting/register/tJEsdO2hrjMrGNH8_ov6k7NZnEqfIEpIBimg