Baker has developed a wide variety of biological safety cabinets designed to meet diverse applications in the life science, clinical, pharmaceutical and industrial laboratory.
Superior Environmental Protection
Because of the critical nature of such laboratory work, Baker products are designed and manufactured to meet specific needs of the workplace, and to operate within a performance envelope which guarantees the widest margin of safety and product protection for the most demanding laboratory conditions.Baker encourages you to learn as much as possible about the capabilities, limitations and appropriate use of specific biosafety cabinets. The adequacy of any containment cabinet for the user’s personal safety should be confirmed by an industrial hygienist or qualified safety officer.
We invite questions and comments by telephone, from our web site, or during personal visits to our manufacturing facility and research laboratories.
In varying degrees, a laminar flow biological safety cabinet is designed to provide three basic types of protection:
Classification is an important consideration in the selection of any biological safety cabinet. Over the years, the scientific community has adopted commonly accepted classification criteria to differentiate containment capabilities and performance attributes. Biological safety cabinets are divided into three classifications. Baker designs and manufactures all of these types of biosafety containment cabinets.
|Class I||1, 2, 3||Low to moderate risk biological agents|
|Class II||1, 2, 3||Low to moderate risk biological agents|
|Class III||4||High risk biological agents|
NSF International (The National Sanitation Foundation) conducts tests on biological safety cabinets to ensure that products achieve minimum standards for cabinet classifications, as devised by NSF. Those standards are defined within NSF/ANSI 49 - 2008, Biosafety Cabinetry: Design, Construction, Performance, and Field Certification.
Cabinets are also required to pass field certification by an independent organization. NSF has established the standards for field tests which define the methods and acceptance criteria that are appropriately applied for determining qualification for field certification of all Class II biological safety cabinets. These field certification procedures are intended to confirm that an installed cabinet evaluated under the current version of the Standard has met all design criteria contained in NSF/ANSI 49.
The Class I biological safety cabinet is designed to provide personnel and environmental protection only.
Baker is internationally recognized for its research and development of Class II products, and offers a complete line of BSC products.
A Class II cabinet must meet requirements for the protection of product, personnel and the environment. This type of cabinet is widely used in clinical, hospital, life science, research and pharmaceutical laboratories.In general, cabinets are classified according to the method by which air volumes are recirculated or exhausted.
The Class II biological safety cabinet has three key features:
Vertical, unidirectional airflow and a front access opening are common to most Class II cabinets. But, because Class II designs permit different airflow patterns, velocities, HEPA air filter position, ventilation rates and exhaust methods, a sub-classification of Type is needed to differentiate Class II BSC designs.
In 2002, NSF restructured the Class II classification system to reflect specific performance and installation attributes.
Cabinets previously classified as Class II, Type A/B3 are now classified as Class II, Type A2. These biosafety cabinets may be exhausted to the room, or connected to a facility exhaust system via a "canopy" connection (NSF recommends a canopy connection for cabinets exhausted to the outdoors). The Class II, Type B3 classification no longer exists for new units.
It is important to understand differences in cabinet Type in order to select the proper cabinet for your application.
|New NSF Classification, Adopted 2002||Previous NSF Classification||General Description|
|A1||Class II, Type A||
|A2||Class II, Type A/B3||
|A2||Class II, Type B3||
|B1||Class II, Type B1||
|B2||Class II, Type B2||
Note: All Baker biological safety cabinets provide containment and protection from airborne particulates for personnel, the work area, and the environment. Depending on the Type and nature of the exhaust configuration, and whether the exhaust system is treated or untreated, this containment and protection can extend to vapors and gases as well.
|Class II Protection||From Particulates||From Vapors and Gases|
|Type A1, Type A2||Personnel, work area (products) and environment||
|Type B1||Personnel, work area (products) and environment||Offers more protection to personnel and the work area the closer the vapor source is located toward rear of work area; (offers protection to the environment if exhausted to treated system)|
|Type B2||Personnel, work area (products) and environment||Offers protection to personnel; (offers protection to environment if exhausted to treated system)|
Biological safety cabinets can be differentiated by a variety of design and performance factors. These include face velocity, air recirculation proportion, contaminated plenum pressure and exhaust function.
HEPA-filtered exhaust air in the Type A1 and A2 cabinet may be recirculated into the room or exhausted to the outdoors through a canopy exhaust connection.
Class II, Type B3 Cabinet
The Class II, Type B3 classification has been eliminated by NSF. This cabinet is now called a Type A2 ducted to the outside..
Class II, Type B1 Cabinet
By definition, Baker Model NCB-C® Class II, Type B1 cabinet meets the ventilation design characteristics established by the National Cancer Institute (NCI) for a General Purpose Clean Air Biological Safety Cabinet (Class II, Type B Safety Cabinet, 6 July 1976). The Baker NCB-C Class II, Type B1 exceeds the NSF Standard with unique design and features offering significant advantages in both particulate and gas/vapor containment.
Biosafety Cabinets with Unique HEPA Air Filtration
Baker has developed the Model NCB-C® to exceed typical Class II, Type B1 criteria, with unique design features proven to enhance containment while inhibiting cross contamination and exposure to chemicals, vapors and gases.
Approximately 40% of the descending air is pulled forward where it mixes with room air entering the perforated front grille. This air passes through the HEPA supply filter directly below the work surface, then is circulated under positive pressure through a duct to the top of the cabinet, then through another HEPA supply filter, where the process is repeated.
Class II, Type B2
Class II, Type B2 cabinets are total exhaust cabinets, widely used in toxicology laboratories and similar applications where chemical effluent is present and clean air is essential.
Class III Total Containment Cabinets
Class III biological safety cabinets are gas-tight, designed for use with high risk biological agents. Class III cabinets provide the highest level of personnel, product and environmental protection. Because of the sensitive nature of most procedures performed within a Class III cabinet, Baker builds each BSC system to exact customer specifications.
Typical applications include: