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Volatile Chemicals and Class II Type A2 “recirculated BSCs: How much is Safe?

Volatile chemicals, while typically used in a fume hood, may sometimes be used in conjunctionwith biological experiments which often require the use of a biosafety cabinet (BSC).  We have derived a series of equations to calculate the internal concentration, maximum amount allowed safely and the time required for the evacuation of any volatile chemical, shown both by calculation and experimentation. 

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