Low-Oxygen Stem Cell Studies: Free E-Book

From the publishers of Stem Cells and Development, this e-book contains curated SC&D articles and educational resources on low-oxygen stem cell studies, as well as how to create the most physiologically-relevant environment for your work.

Physiological normoxia is typically characterized by oxygen levels that are much lower than ambient, often as low as 2% or less. Culturing and working with cells continuously at physiological normoxia, rather than risking any exposure to ambient conditions, prevents abnormal cell interactions and preserves cell viability. Equipment such as tri-gas incubators and hypoxia workstations, as well as protocols for deoxygenating culture media, are often used to simulate normal conditions in the body’s organ systems by providing a low-oxygen incubation and/or workflow environment.The four articles contained in this Stem Cells and Development e-book demonstrate the value of controlling oxygen in stem cell research.

• Endoderm Differentiation: Pimton, Lecht, et al, discuss the importance of the extent of hypoxia and careful timing as components of in vitro differentiation bioprocesses for the differential generation of distal lung epithelial cells from pluripotent progenitors.
• ASCs Induce Tregs: This work by Frazer, et al, suggests that under physiologically relevant low O2conditions, direct contact of human adipose tissue stromal/stem cells with naive CD4+ T cells induces functional iTregs.
• hiPSCs for Tissue Engineering: Kusuma, et al, investigate whether vascular cells derived from human induced pluripotent stem cells exhibit two critical properties to facilitate their use in engineered tissues: extracellular matrix production to confer structure integrity, and growth factor production to facilitate integration.
• Notch Signaling: Moriyama, H., Moriyama, M., et al, show that 5% O2 dramatically increased the glycolysis rate, improved the proliferation efficiency, prevented senescence, and maintained the multipotentency of human adipose tissue-derived multilineage progenitor cells. These effects were not mediated by hypoxia-inducible factor (HIF), but rather by the Notch signaling pathway.

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