Low-Oxygen Stem Cell Studies: Free E-Book

Once again, Baker Ruskinn has sponsored a free, interactive e-book on low-oxygen studies, this time focused on stem cell research.

From the publishers of Stem Cells and Development, this e-book contains curated SC&D articles and educational resources on low-oxygen stem cell studies, as well as how to create the most physiologically-relevant environment for your work.

Physiological normoxia is typically characterized by oxygen levels that are much lower than ambient, often as low as 2% or less. Culturing and working with cells continuously at physiological normoxia, rather than risking any exposure to ambient conditions, prevents abnormal cell interactions and preserves cell viability. Equipment such as tri-gas incubators and hypoxia workstations, as well as protocols for deoxygenating culture media, are often used to simulate normal conditions in the body’s organ systems by providing a low-oxygen incubation and/or workflow environment.The four articles contained in this Stem Cells and Development e-book demonstrate the value of controlling oxygen in stem cell research.

Endoderm Differentiation: Pimton, Lecht, et al, discuss the importance of the extent of hypoxia and careful timing as components of in vitro differentiation bioprocesses for the differential generation of distal lung epithelial cells from pluripotent progenitors.
ASCs Induce Tregs: This work by Frazer, et al, suggests that under physiologically relevant low O2conditions, direct contact of human adipose tissue stromal/stem cells with naive CD4+ T cells induces functional iTregs.
hiPSCs for Tissue Engineering: Kusuma, et al, investigate whether vascular cells derived from human induced pluripotent stem cells exhibit two critical properties to facilitate their use in engineered tissues: extracellular matrix production to confer structure integrity, and growth factor production to facilitate integration.
Notch Signaling: Moriyama, H., Moriyama, M., et al, show that 5% O₂ dramatically increased the glycolysis rate, improved the proliferation efficiency, prevented senescence, and maintained the multipotentency of human adipose tissue-derived multilineage progenitor cells. These effects were not mediated by hypoxia-inducible factor (HIF), but rather by the Notch signaling pathway.